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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in lots of tumors, which leads to chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was uncovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential job in advertising cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually described. Two extra splice variants were claimed but thorough nucleotide sequence details will not be nevertheless available.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide generally has the epitope recognized from the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the best possible effects are...
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This information outlines even more doable optimization of mobile and immunoassay parameters to be certain the very best effects are...
Antibodies sure to the blocking peptide now not bind to your epitope to the focus on protein. By evaluating the staining through the here blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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